1YG2

Structure of the Vibrio cholerae virulence activator AphA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-07-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	77.500, 94.650, 59.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.290 - 2.200
R-factor	0.2331
Rwork	0.233
R-free	0.28000
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.290	2.400
High resolution limit [Å]	2.200	2.200
Number of reflections	11425
Completeness [%]	99.6	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3		MES, magenesium sulphate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 100K
1	VAPOR DIFFUSION, HANGING DROP	6.3		MES, magenesium sulphate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 100K