1YFT
The crystal structure of the catalytic fragment of alanyl-tRNA synthetase in complex wtih glycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-07-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.82653 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.912, 73.912, 172.905 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.230 |
| R-factor | 0.21 |
| Rwork | 0.207 |
| R-free | 0.23900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1riq |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.431 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.083 | 0.440 |
| Number of reflections | 24100 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 298 | PEG 5000 mme, NaCL, Tris-Cl, Tris-base, glycine, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
