1YFG
YEAST INITIATOR TRNA
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL |
| Synchrotron site | SSRL |
| Temperature [K] | 278 |
| Detector technology | FILM |
| Collection date | 1988-01-01 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 113.720, 113.720, 136.541 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| Structure solution method | MIR |
| Starting model (for MR) | 4 A STRUCTURE REPORTED IN REFERENCE 2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.025 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | NUCLIN/PROFFT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.140 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.080 * | |
| Number of reflections | 10688 | |
| <I/σ(I)> | 15.7 | 2.24 |
| Completeness [%] | 95.0 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 301 | Johnson, C.D., (1970) Nature, 226, 1246. * |
| 1 | VAPOR DIFFUSION | 6 | 301 | Johnson, C.D., (1970) Nature, 226, 1246. * |
| 1 | VAPOR DIFFUSION | 6 | 301 | Johnson, C.D., (1970) Nature, 226, 1246. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NH4 SULFATE | ||
| 3 | 1 | 1 | MG SULFATE | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | NA CACODYLATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ammonium sulfate | 1.9 (M) | |
| 2 | 1 | drop | 0.005 (M) | ||
| 3 | 1 | drop | sodium cacodylate | 0.005 (M) | |
| 4 | 1 | drop | spermine HCl | 0.002 (M) | |
| 5 | 1 | drop | tRNA | ||
| 6 | 1 | reservoir | ammonium sulfate | 2.3 (M) |
