1YFE
Crystal structure of apo fumarase C from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-12-04 |
| Detector | XENTRONICS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 121.570, 127.960, 62.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.190 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1FUO (A subunit) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.143 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.070 | 0.310 |
| Number of reflections | 27639 | |
| <I/σ(I)> | 12.963 | 0.943 |
| Completeness [%] | 91.0 | 56 |
| Redundancy | 2.63 | 0.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 286 | PEg 3350, 100 mM MOPS, 50 mM LiSO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 286K |
