1YFD
Crystal structure of the Y122H mutant of ribonucleotide reductase R2 protein from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.040, 84.740, 115.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.460 - 1.900 |
| R-factor | 0.17449 |
| Rwork | 0.173 |
| R-free | 0.20601 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pfr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.353 |
| Data reduction software | XFIT |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.600 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 57178 | |
| <I/σ(I)> | 10.8 | 6.8 |
| Completeness [%] | 99.1 | 98.4 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG 4000, 0.1M MES, 0.4M NACL, 1mM EMTS, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
