1YF9
Structural analysis of Leishmania major ubiquitin conjugating enzyme E2
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97957, 0.95372, 0.97975 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 114.448, 114.448, 139.547 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.18613 |
| Rwork | 0.185 |
| R-free | 0.21039 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.153 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.108 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 54915 | |
| <I/σ(I)> | 13.3 | 1.6 |
| Completeness [%] | 91.7 | 61.17 |
| Redundancy | 6.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 3 M Sodium chloride 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
