1YF6

Structure of a quintuple mutant of photosynthetic reaction center from rhodobacter sphaeroides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-24
Detector	ADSC QUANTUM 4
Spacegroup name	P 31 2 1
Unit cell lengths	139.442, 139.442, 185.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.340 - 2.250
R-factor	0.197
Rwork	0.197
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rzh
RMSD bond length	0.028
RMSD bond angle	2.400
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.340	2.370
High resolution limit [Å]	2.250	2.250
Number of reflections	84139
<I/σ(I)>	6.6	1.8
Completeness [%]	84.8	70.8
Redundancy	3.6	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	POTASSIUM PHOSPHATE, SODIUM CHLORIDE, HAPTANETRIOL, DIOXANE, HAXANETRIOL, LDAO, pH 8.50, VAPOR DIFFUSION, SITTING DROP, temperature 292K