1YEW
Crystal structure of particulate methane monooxygenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.377, 1.280 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 264.140, 264.140, 150.005 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.550 - 2.801 |
| R-factor | 0.27303 |
| Rwork | 0.272 |
| R-free | 0.30157 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.074 |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.874 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 118268 | |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 91.1 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | PEG 8000, zinc acetate, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
