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HUMAN HSP90 GELDANAMYCIN-BINDING DOMAIN, "OPEN" CONFORMATION

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1996-11-11
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1 21 1
Unit cell lengths	53.422, 44.108, 54.079
Unit cell angles	90.00, 115.70, 90.00

Refinement procedure

Resolution	7.000 - 2.200
R-factor	0.189
Rwork	0.189
RMSD bond length	0.010
RMSD bond angle	1.700
Data reduction software	HKL
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.035
Total number of observations	51794 *
Number of reflections	11381
Completeness [%]	97.7
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	17 (mg/ml)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir		0.2 (M)
4	1	reservoir	PEG4000	30 (%)