Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0600 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 97.016, 97.016, 127.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.932 - 2.300 |
| R-factor | 0.22461 |
| Rwork | 0.223 |
| R-free | 0.25780 |
| Structure solution method | SAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.153 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.03) |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.065 | 0.057 | 0.160 |
| Total number of observations | 3337 | 1287 | |
| Number of reflections | 28161 | ||
| Completeness [%] | 89.2 | 99.8 | 41.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | modified microbatch | 7.9 | 313 | 1.2M sodium dihydrogen phosphate, 1.2M potassium dihydrogen sulfate, 0.1M TRIS, pH 7.9, modified microbatch, temperature 313K |
