1YEH
STRUCTURE OF IGG2A FAB FRAGMENT
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE D41A |
| Synchrotron site | LURE |
| Beamline | D41A |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 78.470, 78.470, 158.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.550 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yec |
| RMSD bond length | 0.008 |
| RMSD bond angle | 28.450 * |
| Data reduction software | MOSFLM (5.2) |
| Data scaling software | Agrovata |
| Phasing software | X-PLOR (3.84) |
| Refinement software | X-PLOR (3.84) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.670 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.048 * | 0.170 * |
| Total number of observations | 57600 * | |
| Number of reflections | 18580 | |
| <I/σ(I)> | 11.6 | 4.4 |
| Completeness [%] | 96.0 | 76 |
| Redundancy | 3.1 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | HANGING DROP METHOD. PRECIPITANT: 35% (W/V) PEG 600, 100MM CACODYLATE PH7.5, 40MM ZN ACETATE., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | drop | cacodylate | 100 (mM) | |
| 3 | 1 | reservoir | zinc acetate | 40 (mM) | |
| 4 | 1 | reservoir | PEG600 | 35 (%(w/v)) |
