1YEE

STRUCTURE OF A CATALYTIC ANTIBODY, IGG2A FAB FRAGMENT (D2.5)

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE D41A
Synchrotron site	LURE
Beamline	D41A
Temperature [K]	280
Detector technology	IMAGE PLATE
Collection date	1995-09
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	67.930, 76.980, 45.800
Unit cell angles	90.00, 94.68, 90.00

Refinement procedure

Resolution	7.000 - 2.200
R-factor	0.204
Rwork	0.204
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yec
RMSD bond length	0.013
RMSD bond angle	28.790 *
Data reduction software	MOSFLM (5.2)
Data scaling software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.200
Rmerge	0.083
Number of reflections	21342
Completeness [%]	95.0
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.6		pH 5.6