1YE8
Crystal Structure of THEP1 from the hyperthermophile Aquifex aeolicus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97625, 0.979802 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.870, 64.240, 39.620 |
| Unit cell angles | 90.00, 105.23, 90.00 |
Refinement procedure
| Resolution | 18.210 - 1.400 |
| R-factor | 0.17351 |
| Rwork | 0.172 |
| R-free | 0.20749 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.334 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.210 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.084 | 0.397 |
| Number of reflections | 33181 | |
| <I/σ(I)> | 11.67 | 3.49 |
| Completeness [%] | 95.3 | 90.6 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | PEG 3350, potassium dihydrogenphosphate, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
