1YDW

X-RAY STRUCTURE OF GENE PRODUCT FROM ARABIDOPSIS THALIANA AT4G09670

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-24
Detector	MAR CCD 165 mm
Wavelength(s)	0.97927
Spacegroup name	P 21 21 21
Unit cell lengths	58.197, 107.663, 129.748
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.060 - 2.488
Rwork	0.218
R-free	0.28254
Structure solution method	SAD
RMSD bond length	0.022
RMSD bond angle	2.050
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.06)
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	33.749	50.000	2.590
High resolution limit [Å]	2.488	5.380	2.488
Rmerge	0.107	0.077	0.298
Total number of observations		3144	2439
Number of reflections	29008
<I/σ(I)>	13.245		4.321
Completeness [%]	98.3	99.4	84.5
Redundancy	13.4		8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	10 MG/ML PROTEIN, 15% PEG4K, 0.168 M SODIUM CHLORIDE, 0.050 M PIPES, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 6.5