1YDG
Crystal Structure of Trp repressor binding protein WrbA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | .9793 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 121.611, 121.611, 207.933 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.18877 |
| Rwork | 0.186 |
| R-free | 0.23600 * |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.182 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.086 | 0.290 |
| Number of reflections | 116736 | |
| <I/σ(I)> | 13 | 5.6 |
| Completeness [%] | 97.0 | 99.8 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 20 * | 2.6M AmSulfate, 20% Glycerol, pH 4.5, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 27.7 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | glycerol | 10 (%) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.6 (M) | |
| 6 | 1 | reservoir | glycerol | 20 (%) | |
| 7 | 1 | reservoir | dithiothreitol | 5 (mM) | pH4.5 |
