1YD9

1.6A Crystal Structure of the Non-Histone Domain of the Histone Variant MacroH2A1.1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	298
Detector technology	CCD
Collection date	2002-05-25
Detector	ADSC QUANTUM 315
Wavelength(s)	1.074853
Spacegroup name	P 21 21 21
Unit cell lengths	83.114, 89.791, 95.679
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.600
Rwork	0.235
R-free	0.26000
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.261
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.670
High resolution limit [Å]	1.600	1.600
Number of reflections	91017
Completeness [%]	96.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	292	28% PEG 2000, 0.2M Ammonium Sulphate, 0.1M Sodium Acetate, pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 292K
1	VAPOR DIFFUSION, SITTING DROP	5.9	292	28% PEG 2000, 0.2M Ammonium Sulphate, 0.1M Sodium Acetate, pH 5.9, VAPOR DIFFUSION, SITTING DROP, temperature 292K