1YCY
Conserved hypothetical protein Pfu-1806301-001 from Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 82.958, 82.958, 189.782 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| Rwork | 0.281 |
| R-free | 0.31100 |
| Structure solution method | SAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.343 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.03) |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.770 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.089 | 0.294 |
| Number of reflections | 11111 | |
| Completeness [%] | 95.0 | 61.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 291 | 20% w/v PEG 3000, 0.1M sodium acetate, 0.01M NAD, pH 4.5, modified microbatch, temperature 291K, pH 4.50 |
