1YCQ
XENOPUS LAEVIS MDM2 BOUND TO THE TRANSACTIVATION DOMAIN OF HUMAN P53
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-01 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 84.960, 84.960, 63.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.300 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.25300 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.390 |
| Data reduction software | HKL |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.300 * |
| Rmerge | 0.055 |
| Total number of observations | 15953 * |
| Number of reflections | 6257 |
| Completeness [%] | 97.0 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 16 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MES | 25 (mM) | |
| 2 | 1 | drop | 250 (mM) | ||
| 3 | 1 | drop | DTT | 5 (mM) | |
| 4 | 1 | drop | MDM2 | 1.5 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 45 (%sat) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 7 | 1 | reservoir | DTT | 5 (mM) |
