1YCO

Crystal structure of a branched-chain phosphotransacylase from Enterococcus faecalis V583

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-06
Detector	ADSC QUANTAM Q315
Wavelength(s)	0.9790
Spacegroup name	P 43 21 2
Unit cell lengths	61.165, 61.165, 317.995
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.750 - 2.400
R-factor	0.2321
Rwork	0.232
R-free	0.28300
Structure solution method	SAD
Starting model (for MR)	Model built based on a solvent flattened experimental electron density map
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	44872
<I/σ(I)>	31.7
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	18.8% PEG 8000, 0.05M Potassium Phosphate Monobasic, 5% Ethylene Glycol, pH 7., VAPOR DIFFUSION, HANGING DROP, temperature 291K