1YCN
X-RAY STRUCTURE OF ANNEXIN FROM ARABIDOPSIS THALIANA GENE AT1G35720
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-15 |
| Detector | APS-1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 75.543, 96.936, 226.878 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.120 - 2.510 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n00 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP (8.2.01version5.0:04/07/04) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.125 | 2.570 |
| High resolution limit [Å] | 2.508 | 2.510 |
| Rmerge | 0.063 | 0.231 |
| Number of reflections | 27985 | |
| <I/σ(I)> | 12.618 | 4.589 |
| Completeness [%] | 95.4 | 76.9 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 293 | 10 MG/ML PROTEIN, 15% PEG4K, 10% MPD, 0.100 M HEPPS, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 8.00 |
