1YCF
Oxidized (di-ferric) FprA from Moorella thermoacetica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-11-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 159.670, 159.670, 276.798 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.710 - 3.000 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.050 | |
| Number of reflections | 71781 | |
| Completeness [%] | 96.7 | 84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6.5 | 298 | 10% PEG 3000, 200 mM zinc acetate, sodiumcacodylate pH 6.5, LIQUID DIFFUSION, temperature 25K |
