1YCE

Structure of the rotor ring of F-type Na+-ATPase from Ilyobacter tartaricus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-07
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9756
Spacegroup name	P 1 21 1
Unit cell lengths	147.700, 140.000, 153.000
Unit cell angles	90.00, 118.40, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.20282
Rwork	0.201
R-free	0.24595
RMSD bond length	0.021
RMSD bond angle	1.910
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.183	0.635
Number of reflections	198822
<I/σ(I)>	10	3.3
Completeness [%]		95.2
Redundancy	10	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	290	15% PEG400, 0.1M NaAc, pH 4.5, VAPOR DIFFUSION, temperature 290K