1YCD
Crystal structure of yeast FSH1/YHR049W, a member of the serine hydrolase family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.133, 53.329, 64.271 |
| Unit cell angles | 102.85, 90.04, 112.47 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.16746 |
| Rwork | 0.166 |
| R-free | 0.20217 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.283 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 57009 | |
| <I/σ(I)> | 14.6 | 3.7 |
| Completeness [%] | 94.8 | 91.2 |
| Redundancy | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 27% PEG 4K, 0.1M MES, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
