1YCA

DISTAL POCKET POLARITY IN LIGAND BINDING TO MYOGLOBIN: DEOXY AND CARBONMONOXY FORMS OF A THREONINE68 (E11) MUTANT INVESTIGATED BY X-RAY CRYSTALLOGRAPHY AND INFRARED SPECTROSCOPY

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	124.200, 42.500, 92.100
Unit cell angles	90.00, 92.20, 90.00

Refinement procedure

Resolution	8.000 - 2.200 *
R-factor	0.189
RMSD bond length	0.020
RMSD bond angle	0.054
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.087 *
Number of reflections	17828 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium phosphate	100 (mM)
2	1	drop	ammonium sulfate	70-80 (%)
3	1	drop	protein	5-6 (mg/ml)
4	1	drop	sodium dithionite		50-fold molar excess