1YC6

Crystallographic Structure of the T=1 Particle of Brome Mosaic Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2000-07-02
Detector	SDMS
Wavelength(s)	1.1
Spacegroup name	P 43 2 2
Unit cell lengths	194.770, 194.770, 428.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.260 - 2.900
R-factor	0.22
Rwork	0.219
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1js9
RMSD bond length	0.010
RMSD bond angle	1.420
Data reduction software	SDMS
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.300	3.000
High resolution limit [Å]	2.900	2.900
Number of reflections	181726
<I/σ(I)>	8.07	1.93
Completeness [%]	99.5	96.5
Redundancy	18.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	sodium malonate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K