1YBY
Conserved hypothetical protein Cth-95 from Clostridium thermocellum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.915, 55.476, 88.462 |
| Unit cell angles | 90.00, 120.84, 90.00 |
Refinement procedure
| Resolution | 27.735 - 1.950 |
| R-factor | 0.22883 |
| Rwork | 0.228 |
| R-free | 0.27040 |
| Structure solution method | SAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.408 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.03) |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
| Rmerge | 0.083 | 0.055 | 0.541 |
| Total number of observations | 4183 | 3863 | |
| Number of reflections | 39625 | ||
| Completeness [%] | 97.0 | 99.7 | 94.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop, vapor diffusion | 7.6 | 291 | 1.5M dipotassium hydrogen phosphate, 0.1M HEPES, pH 7.6, sitting drop, vapor diffusion, temperature 291K |
