1YBT
MYCOBACTERIUM TUBERCULOSIS ADENYLYL CYCLASE, RV1900C CHD
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-01 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.979290, 0.979399, 0.956668 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 90.704, 44.386, 80.425 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 2.310 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.23000 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.081 | 0.369 |
| Number of reflections | 28083 | |
| <I/σ(I)> | 12.1 | 1.1 |
| Completeness [%] | 99.1 | 97.3 |
| Redundancy | 2.55 | 2.19 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | HEPES, PEG 1000, NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
