1YBF
Crystal structure of AMP nucleosidase from Bacteroides thetaiotaomicron VPI-5482
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 108.120, 108.120, 160.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| Rwork | 0.228 |
| R-free | 0.26410 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.326 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (& SHARP) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.087 | 0.350 |
| Number of reflections | 26147 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 97.0 | 95 |
| Redundancy | 6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.5 M Magnesium formate, Bis-Tris buffer, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
