1YBA
The active form of phosphoglycerate dehydrogenase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-12 |
| Detector | SBC |
| Wavelength(s) | 0.9537,0.9795,0.9797 |
| Spacegroup name | P 43 |
| Unit cell lengths | 76.159, 76.159, 354.144 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.240 |
| R-factor | 0.18891 |
| Rwork | 0.186 |
| R-free | 0.23512 |
| Structure solution method | MAD |
| Starting model (for MR) | Resolve PDB output based on MAD phasing |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.692 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.089 | |
| Number of reflections | 92508 | |
| <I/σ(I)> | 20.2 | 2.3 |
| Completeness [%] | 96.3 | 77.3 |
| Redundancy | 12.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | 1.18 M to 1.25 M AmSO4, 100 mM KPO4, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
