1YB4
Crystal Structure of the Tartronic Semialdehyde Reductase from Salmonella typhimurium LT2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-12 |
Detector | SBC-2 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 98.335, 134.255, 48.216 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.350 - 2.400 |
R-factor | 0.236 |
Rwork | 0.229 |
R-free | 0.30400 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.350 | 2.530 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.132 | |
Number of reflections | 24924 | |
<I/σ(I)> | 8.7 | |
Completeness [%] | 97.6 | 98.4 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | PEG8000, TrisHCl, magnesium chloride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | PEG8000, TrisHCl, magnesium chloride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | PEG8000, TrisHCl, magnesium chloride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |