1YB2
Structure of a putative methyltransferase from Thermoplasma acidophilum.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-10-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97944 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.316, 60.557, 65.841 |
| Unit cell angles | 90.00, 105.12, 90.00 |
Refinement procedure
| Resolution | 27.700 - 2.010 |
| R-factor | 0.20149 |
| Rwork | 0.199 |
| R-free | 0.24718 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.301 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.700 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 18240 | |
| <I/σ(I)> | 2.22 | |
| Completeness [%] | 98.7 | 89 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | ammonium formate, PEG 3350, sucrose, glycerol, isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
