1YAG
STRUCTURE OF THE YEAST ACTIN-HUMAN GELSOLIN SEGMENT 1 COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.662, 68.099, 54.598 |
| Unit cell angles | 90.00, 102.55, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.1915 * |
| Rwork | 0.193 |
| R-free | 0.23040 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RABBIT SKELETAL MUSCLE ACTIN-HUMAN GELSOLIN SEGMENT 1 COMPLEX |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.532 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.081 | 0.360 |
| Number of reflections | 49913 | |
| Completeness [%] | 100.0 | 99.9 * |
| Redundancy | 3.77 * | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.4-1.7 (M) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | |
| 3 | 1 | reservoir | Mg-ATP | 2 (mM) | |
| 4 | 1 | drop | gelositin | 1:1.25 stoichiometry | |
| 5 | 1 | drop | Tris-HCl | 2 (mM) | |
| 6 | 1 | drop | ATP | 2 (mM) | |
| 7 | 1 | drop | 4 (mM) | ||
| 8 | 1 | drop | EGTA | 0.5 (mM) | |
| 9 | 1 | drop | 1 (mM) |
