1Y9T
Crystal structure of a type III secretion system protein complexed with the lipid, 1-monohexanoyl-2-hydroxy-sn-glycero-3-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-06-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.036, 49.721, 29.520 |
| Unit cell angles | 90.00, 101.99, 90.00 |
Refinement procedure
| Resolution | 42.400 - 1.870 |
| R-factor | 0.20843 |
| Rwork | 0.206 |
| R-free | 0.24907 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1y9l |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.473 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.400 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 9460 | |
| <I/σ(I)> | 34 | 6.2 |
| Completeness [%] | 96.4 | 93.6 |
| Redundancy | 4.6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | (NH3)2SO4 LiSO4 NaCHOCH3, 1-monohexanoyl-2-hydroxy-sn-glycero-3-phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
