1Y9L
The X-ray structure of an secretion system protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2003-06-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0, 0.8981, 0.9794, 0.9796 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.844, 49.683, 29.487 |
| Unit cell angles | 90.00, 102.27, 90.00 |
Refinement procedure
| Resolution | 20.261 - 1.500 |
| R-factor | 0.22028 |
| Rwork | 0.220 |
| R-free | 0.25071 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.468 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.261 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 18209 | |
| <I/σ(I)> | 16.5 | 3 |
| Completeness [%] | 96.5 | 100 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | (NH4)2SO4, LiSO4, NaCH3CO2, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
