1Y9B

Structure of Conserved Putative Transcriptional Factor from Vibrio cholerae O1 biovar eltor str. N16961

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	150
Detector technology	CCD
Collection date	2004-10-11
Detector	SBC-2
Wavelength(s)	.97944
Spacegroup name	P 21 21 2
Unit cell lengths	66.220, 72.732, 48.469
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.970 - 2.200
R-factor	0.22747
Rwork	0.225
R-free	0.28598
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.460
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.970	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	12046
Completeness [%]	97.4	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	298	1.1M Sodium malonate, 0.1M Hepes, 0.5% w/v jeffamine ED-2001, pH 7.0, VAPOR DIFFUSION, temperature 298K