1Y96

crystal structure of the Gemin6/Gemin7 heterodimer from the human SMN complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-24
Detector	ADSC QUANTUM 4
Wavelength(s)	1.07810
Spacegroup name	P 42 21 2
Unit cell lengths	136.011, 136.011, 81.515
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.002
R-factor	0.21869
Rwork	0.217
R-free	0.25177
Structure solution method	MAD
RMSD bond length	0.019
RMSD bond angle	1.283
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.054
High resolution limit [Å]	2.002	2.002
Number of reflections	49487
Completeness [%]	95.6	95.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	291	sodium acetate, MPD, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	4.7	291	sodium acetate, MPD, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 291K