1Y8T
Crystal Structure of RV0983 from Mycobacterium tuberculosis- Proteolytically active form
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-30 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979, 0.94181, 0.97923 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.584, 89.067, 69.408 |
| Unit cell angles | 90.00, 97.55, 90.00 |
Refinement procedure
| Resolution | 76.700 - 2.000 |
| R-factor | 0.22931 |
| Rwork | 0.227 |
| R-free | 0.28027 |
| Structure solution method | MAD |
| Starting model (for MR) | CRYSTAL STRUCTURE OF RV1223 (STRUCTURE of RV1223 has been SOLVED at HOME TO BE SUBMITTED TO PDB SOON) |
| RMSD bond length | 0.035 |
| RMSD bond angle | 3.164 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.700 | 1.990 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.050 | 0.900 |
| Number of reflections | 76977 | |
| <I/σ(I)> | 24.12 | 0.87 |
| Completeness [%] | 93.8 | 76 |
| Redundancy | 7.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
