1Y8P
Crystal structure of the PDK3-L2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-08-17 |
Detector | ADSC QUANTUM 310 |
Wavelength(s) | 0.97 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 120.904, 120.904, 240.085 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.630 |
R-factor | 0.20495 |
Rwork | 0.204 |
R-free | 0.23020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDK2 PDB entry 1JM6 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.731 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.710 |
High resolution limit [Å] | 2.620 | 2.620 |
Rmerge | 0.059 | 0.542 |
Number of reflections | 31043 | |
<I/σ(I)> | 30 | 2.1 |
Completeness [%] | 98.4 | 97.2 |
Redundancy | 7.5 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | soudium citrate, sodium potassium phosphate, sodium chrolide, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |