1Y8C
Crystal structure of a S-adenosylmethionine-dependent methyltransferase from Clostridium acetobutylicum ATCC 824
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-06 |
| Detector | ADSC QUANTAM Q315 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 67.918, 67.918, 134.499 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.270 - 2.500 |
| R-factor | 0.2211 |
| Rwork | 0.221 |
| R-free | 0.27500 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 23695 | |
| <I/σ(I)> | 22.7 | |
| Completeness [%] | 98.8 | 96.9 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 3.6M Ammonium Sulfate, 0.1M Potassium Phosphate Monobasic, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
