1Y8A

Structure of gene product AF1437 from Archaeoglobus fulgidus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	110
Detector technology	CCD
Collection date	2004-10-10
Detector	CUSTOM-MADE
Wavelength(s)	0.97944
Spacegroup name	P 21 21 2
Unit cell lengths	74.900, 84.027, 44.175
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.640 - 1.400
R-factor	0.18479
Rwork	0.183
R-free	0.22270
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.159
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.640	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	53385
Completeness [%]	96.0	70.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	HEPES, MgCl2, PEG 3350, Glycerol , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	HEPES, MgCl2, PEG 3350, Glycerol , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	HEPES, MgCl2, PEG 3350, Glycerol , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K