1Y89
Crystal Structure of devB protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-26 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 85.078, 85.078, 181.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.420 - 2.000 |
| R-factor | 0.16076 |
| Rwork | 0.159 |
| R-free | 0.19434 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.420 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.092 | 0.501 |
| Number of reflections | 52566 | |
| <I/σ(I)> | 19.55 | 3.44 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.6 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 40% PEG-300, HEPES pH 7.5, 0.2 M NaCl, VAPOR DIFFUSION, temperature 293K |
