1Y88

Crystal Structure of Protein of Unknown Function AF1548

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-29
Detector	CUSTOM-MADE
Wavelength(s)	0.9791
Spacegroup name	P 63 2 2
Unit cell lengths	124.225, 124.225, 62.982
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.850
R-factor	0.20321
Rwork	0.202
R-free	0.23290
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.461
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.920
High resolution limit [Å]	1.850	1.850
Number of reflections	46443
Completeness [%]		100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.2M (NH4)2SO4, 15% PEG 3350, 2% PEG 400, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K