1Y81
Conserved hypothetical protein Pfu-723267-001 from Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-26 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 62 |
| Unit cell lengths | 79.146, 79.146, 36.402 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.519 - 1.701 |
| R-factor | 0.22334 |
| Rwork | 0.223 |
| R-free | 0.24500 |
| Structure solution method | SAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.325 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.06) |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.650 |
| High resolution limit [Å] | 1.590 | 3.430 | 1.590 |
| Rmerge | 0.054 | 0.039 | 0.371 |
| Total number of observations | 1857 | 354 | |
| Number of reflections | 14818 | ||
| Completeness [%] | 83.1 | 99.8 | 20.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | modified microbatch | 291 | 0.3M sodium thiocyanate, 35% w/v PEG 3350, modified microbatch, temperature 291K |
