1Y7Y
High-resolution crystal structure of the restriction-modification controller protein C.AhdI from Aeromonas hydrophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.904989 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 24.403, 57.246, 46.125 |
| Unit cell angles | 90.00, 98.89, 90.00 |
Refinement procedure
| Resolution | 18.400 - 1.690 |
| Rwork | 0.169 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Mutant I58M of C.AhdI SeMet MAD to 2.5A |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.524 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.570 | 1.750 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.048 | 0.215 |
| Number of reflections | 14302 | |
| <I/σ(I)> | 8 | 4.1 |
| Completeness [%] | 95.7 | 95.7 |
| Redundancy | 9.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | PEG 6000, IMIDAZOLE-MALATE, 2-METHYL-2,4-PENTANEDIOL, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
