1Y7R
1.7 A Crystal Structure of Protein of Unknown Function SA2161 from Meticillin-Resistant Staphylococcus aureus, Probable Acetyltransferase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97948, 0.97959 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.168, 44.171, 71.408 |
| Unit cell angles | 90.00, 100.73, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.17419 |
| Rwork | 0.172 |
| R-free | 0.21852 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.625 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.780 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 25772 | |
| Completeness [%] | 92.9 | 80.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | mono-ammonium dihydrogen phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
