1Y7P
1.9 A Crystal Structure of a Protein of Unknown Function AF1403 from Archaeoglobus fulgidus, Probable Metabolic Regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-07 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 95.709, 95.709, 167.551 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.340 - 1.900 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.25500 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.495 |
| Number of reflections | 102527 | |
| <I/σ(I)> | 22.6 | 1.534 |
| Completeness [%] | 87.4 | 85.2 |
| Redundancy | 6.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.2 M K/Na Tartr., 20% PEG3350, 0.05M Tris buffer, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
