1Y7O
The structure of Streptococcus pneumoniae A153P ClpP
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9675,0.9950 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 105.650, 105.650, 236.192 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.500 - 2.510 |
| R-factor | 0.19222 |
| Rwork | 0.186 |
| R-free | 0.24814 |
| Structure solution method | SAD + MR |
| Starting model (for MR) | 1tyf |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.302 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNX (2000.1) |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 47508 | |
| <I/σ(I)> | 16.6 | 2.7 |
| Completeness [%] | 71.1 | |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | PEG 400, Calcium acetate, cacodylate, pH 7.0, VAPOR DIFFUSION, temperature 293K |
