1Y7M
Crystal Structure of the B. subtilis YkuD protein at 2 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 77.3 |
| Detector technology | CCD |
| Collection date | 2004-04-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97946, 0.97919, 0.97178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.276, 63.910, 93.679 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.050 |
| R-factor | 0.214 |
| Rwork | 0.211 |
| R-free | 0.27000 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.441 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.057 | 0.459 |
| Number of reflections | 21805 | |
| <I/σ(I)> | 15.5 | 2.8 |
| Completeness [%] | 99.1 | 99 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.9 | 294.1 | PEG 3350, acetate, lithium sulfate, cadmium chloride, pH 4.9, VAPOR DIFFUSION, SITTING DROP, temperature 294.1K, pH 4.90 |
