1Y7L
O-Acetylserine Sulfhydrylase Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 125 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-26 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 |
| Unit cell lengths | 112.569, 112.569, 45.799 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.210 - 1.550 |
| R-factor | 0.1971 |
| Rwork | 0.197 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oas |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.210 | 1.650 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.071 | |
| Number of reflections | 40063 | |
| Completeness [%] | 95.9 | 88.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | ammonium sulfate, PEG 400, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
