1Y7B
BETA-D-XYLOSIDASE, A FAMILY 43 GLYCOSIDE HYDROLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.480, 136.150, 183.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.600 |
| R-factor | 0.158 |
| Rwork | 0.157 |
| R-free | 0.20600 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.439 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.500 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.087 | 0.398 |
| Number of reflections | 290830 | |
| <I/σ(I)> | 9.5 | 1.8 |
| Completeness [%] | 94.0 | 86.5 |
| Redundancy | 7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1 M HEPES, 0.2 M Ammonium Sulfate, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
